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an Base and Prime Editing Reduce the Cancer Risk Linked to Conventional CRISPR?” of master’s level

Topic Outline Assessment Task 2A

What is the topic? CRISPR.

What is the focus? The balance between the benefit of reduced off-target sites that minimise the cancer risk associated with conventional CRISPR and the generation of bystander mutations.

What is the controversy? The uncertainty surrounding whether the benefits of reduced off-target sites offered by the base and prime editors outweigh the concerns related to the notable generation of bystander mutations.

What is the title? Can Base and Prime Editing Reduce the Cancer Risk Linked to Conventional CRISPR?

 

Can Base and Prime Editing Reduce the Cancer Risk Linked to Conventional CRISPR? 

Abstract (250 words, 1 paragraph)

  • Description of the controversy – uncertainty on whether the benefits of reduced off-target sites offered by the base and prime editors outweigh the concerns related to the notable generation of bystander mutations.
  • The aim of the review – to critically analyse the balance between the benefit of reduced off-target sites and the risk associated with the generation of bystander mutations.
  • Key findings and insights of the review.
  • Overall conclusion
  1. Introduction
  • An overview of CRISPR gene-editing technology, its impact on gene therapy, and the introduction to the cancer risk associated with off-target sites of conventional techniques.
  • Introduction to the newer base and prime editing techniques of CRISPR and their potential benefits, particularly their promise of reducing off-target sites.
  • State the controversy and focus of the critical review: uncertainty regarding whether the benefits of reduced off-target sites outweigh concerns about bystander mutations.
  • State the aim and scope of the critical review: to critically analyse the balance between the benefit of reduced off-target sites and the risk associated with bystander mutations and highlight the issue’s significance in the context of CRISPR applications and their potential impact on human health.
  1. CRISPR Gene-Editing Technology
  • Mechanism of conventional CRISPR gene editing.
  • Disadvantages of using conventional CRISPR editing tools with a primary focus on its off-target effects associated with cancer.
  • Challenges in the management of off-target effects
  • Discuss relevant literature.
  1. Base and Prime Editing CRISPR Technique

3.1 Base Editing

  • Explain the base editing process and its differences from conventional CRISPR.
  • Present evidence supporting its effectiveness in reducing off-target sites.
  • Discuss concerns related to the generation of bystander mutations.
  • Evaluate concerns related to the generation of bystander mutations with relevant studies and findings.

3.2 Prime Editing

  • How it works and how it is different from conventional CRISPR techniques.
  • How it can overcome off-target effects.
  • Evaluate concerns related to the generation of bystander mutations with relevant studies and findings.

3.3 Uncertainty in Base and Prime Editing

  • Summarise findings from the critical analysis.
  • Address any gaps in current knowledge or areas.
  • Explore the implications of uncertainty on the overall balance between benefits and risks.
  1. Conclusion (1 paragraph)
  • Restate the aim and controversy.
  • Summarise key findings, insights and any conclusions made.
  • Suggestions to future research directions regarding limitations and inconsistencies across studies.
  1. References

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